Part:BBa_K3738010
MS2 Bacteriophage Capsid Protein with Maturation Protein A
The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as one copy of maturation protein A (BBa_K3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo.
The first biobrick in this composite part includes the MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide (BBa_K2683012) under the control of an strong arabinose-inducible promoter (BBa_K206000), as well as the RBS BBa_B0034 and double terminator BBa_B0015. The second biobrick contains maturation protein A (BBa_K3738000) under the control of weak constitutive promoter BBa_J23114, as well as the RBS BBa_B0034 and double terminator BBa_B0015. Given the high ratio of capsid protein to maturation protein A required, this design will result in higher production of the capsid protein, which can be induced once there is sufficient cell growth, resulting in self-assembly of functional MS2 PLPs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1490
Illegal NheI site found at 1513 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal XhoI site found at 1075 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1609
- 1000COMPATIBLE WITH RFC[1000]
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